ZO1 Polyclonal Antibody

Catalog No.:AB0054-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal to TJP1 tight junction protein 1 (TJP1). TJP1 is located on a cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.

Alternative names:
ZO-1 and zona occludens protein 1 antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.

Specificity: Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                            1:500-1:2,000
Immunofluorescence                              1:50-1:250
Immunohistochemistry (paraffin)           1:50-1:200
Immunohistochemistry (frozen)             1:50-1:200

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Pelkonen L, Sato K, Reinisalo M, et al. Mol Pharm. 2017 Mar 6. Suplemental Material. PMID:28112518



For research use only, not for diagnostic use.