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Vimentin Polyclonal Antibody

Catalog No.:AB252253-100

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 200 µg

Description: Goat polyclonal antibody to Vimentin. VIM is a member of the intermediate filament family. The cytoskeleton is made by these intermediate filaments, along with actin microfilaments and microtubules. VIM is responsible for integrity of the cytoplasm, stabilization of the cytoskeletal interactions and maintaining cell shape. This protein controls the transport of LDL-derived cholesterol from a lysosome to the site of esterification and is also involved in the immune response. It functions as an organizer of a number of critical proteins involved in migration, attachment and cell signaling.

Alternative names
: CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM antibody.

Form: Polyclonal antibody supplied as a 100 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Recombinant peptide derived from within residues 390 aa to the C-terminus of human VIM produced in E. coli.

Specificity: Detects endogenous levels of VIM by Western blot.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                        1:500-1:5,000
Immunofluorescence                                 ND
Immunohistochemistry (paraffin)               ND
Immunohistochemistry (frozen)                 ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat +++ ND ND ND
mouse +++ ND ND ND
canine +++ ND ND ND
monkey +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References





For research use only, not for diagnostic use