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Venus Polyclonal Antibody

Catalog No.:AB2166-100

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 300 µg

Description: Goat polyclonal antibody to Venus. Venus is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies.

Alternative names:
GFP, Green fluorescent protein antibody.

Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant GFP peptide from the jellyfish aequorea victoria produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins.

Reactivity: Venus, GFP, EGFP and YFP from Aequorea victoria.

Usage: Western blot                    1:500-1:2,000
Immunofluorescence                      1:50-1:1,000
Immunohistochemistry (paraffin)    1:50-1:1,000
Immunohistochemistry (frozen)      1:50-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References




For research use only, not for diagnostic use