Venus Polyclonal Antibody
Catalog No.:AB2166-100
Price / Unit: EUR 320.00
Source: Goat
Quantity/unit size: 300 µg
Description: Goat polyclonal antibody to Venus. Venus is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies.
Alternative names: GFP, Green fluorescent protein antibody.
Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Immunogen: Purified recombinant GFP peptide from the jellyfish aequorea victoria produced in E. coli.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins.
Reactivity: Venus, GFP, EGFP and YFP from Aequorea victoria.
Usage: Western blot 1:500-1:2,000
Immunofluorescence 1:50-1:1,000
Immunohistochemistry (paraffin) 1:50-1:1,000
Immunohistochemistry (frozen) 1:50-1:1,000
Reactivity chart
| Sample | Western Blot | Immuno-fluorescence | Immuno-Histochemistry (p) | Immuno-Histochemistry (f) | transfected cells | +++ | +++ | +++ | +++ |
|---|
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.
References
For research use only, not for diagnostic use
