Catalog No.:AB0096-500
Price / Unit: EUR 450.00
Source: Goat
Quantity/unit size: 1.5 mg
Description: Goat polyclonal antibody to V5 epitope. V5 peptide corresponds to amino acid residues GKPIPNPLLGLDST (95-108 aa) of the P and V proteins of the Paramyxovirus of simian virus SV5.
Alternative names: GKPIPNPLLGLDST epitope, V5 tag antibody.
Form: Polyclonal antibody supplied as a 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Immunogen: Purified recombinant peptide derived from within residues 95-108 aa of of the P/V proteins of the Paramyxovirus SV5 produced in E. coli.
Specificity: Reacts specifically with V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
Reactivity: Reacts against V5-tagged recombinant fusion proteins.
Usage: Western blot 1:500-1:5,000
Immunofluorescence 1:500-1:2,000
Immunohistochemistry (paraffin) ND
Immunohistochemistry (frozen) ND
Reactivity chart
Sample | Western Blot | Immuno-fluorescence | Immuno-Histochemistry (p) | Immuno-Histochemistry (f) | transfected cells | +++ | ND | ND | ND |
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Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.

References
1. Catarino S, Ribeiro-Rodrigues TM, Sa Ferreira R, et al. Cells 2020 Apr. PMID: 32272685
2. Calado J, Santos AR, Aires I, et al. FEBS Lett 2018 Aug. PMID:30156268
For research use only, not for diagnostic use