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Ubiquitin Polyclonal Antibody

Catalog No.:AB0144-200

Price / Unit: EUR 300.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to Ubiquitin. Ubiquitin is a highly conserved of about 8.5 kDa regulatory protein expressed in all eukaryotic tissues. Its function is labelling of proteins for degradation through ubiquitin proteasome system. In order to perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and a complex of degradative enzymes then recognizes the ubiquitinated complex.

Alternative names:
HMG20, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant human ubiquitin (MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG) produced in E. coli.

Specificity: Using the recombinant human ubiquitin gives a positive signal by Western blot.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                          1:250-1:2,000
Immunofluorescence                                  ND
Immunohistochemistry (paraffin)                ND
Immunohistochemistry (frozen)                  ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat +++ ND ND ND
mouse +++ ND ND ND
canine +++ ND ND ND
monkey +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References




For research use only, not for diagnostic use