Tubulin Alpha4A Chain Polyclonal Antibody
Catalog No.:AB0046-500
Price / Unit: EUR 450.00
Source: Goat
Quantity/unit size: 1 mg
Description: Goat polyclonal antibody to alpha tubulin. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.
Alternative names: TUBA1, TUBA4A antibody
Form: Polyclonal antibody supplied as a 500 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Immunogen: Purified recombinant peptide derived from within residues 400 aa to the C-terminus of human alpha tubulin produced in E. coli.
Specificity: This antibody gives a positive signal in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.
Usage: Western blot 1:250-1:2,000
Immunofluorescence 1:50-1:200
Immunohistochemistry (paraffin) ND
Immunohistochemistry (frozen) ND
Reactivity chart
| Sample | Western Blot | Immuno-fluorescence | Immuno-Histochemistry (p) | Immuno-Histochemistry (f) | human | +++ | +++ | ND | ND | rat | +++ | +++ | ND | ND | mouse | +++ | +++ | ND | ND | canine | +++ | +++ | ND | ND | monkey | +++ | +++ | ND | ND |
|---|
Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.
References
1. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One. 2019 Oct. PMID:31613922
2. Goncalves SMM, PhD Thesis, University of Minho - Portugal 2015
For research use only, not for diagnostic use
