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Tubulin Alpha4A Chain Polyclonal Antibody

Catalog No.:AB0046-200

Price / Unit: EUR 300.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal antibody to alpha tubulin. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.

Alternative names: TUBA1, TUBA4A antibody.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 400 aa to the C-terminus of human alpha tubulin produced in E. coli.

Specificity: This antibody gives a positive signal in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                           1:250-1:2,000
Immunofluorescence                               1:50-1:200
Immunohistochemistry (paraffin)                  ND
Immunohistochemistry (frozen)                    ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ ND ND
rat +++ +++ ND ND
mouse +++ +++ ND ND
canine +++ +++ ND ND
monkey +++ +++ ND ND

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Goncalves SMM, PhD Thesis, University of Minho - Portugal 2015


For research use only, not for diagnostic use