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TNF Polyclonal Antibody

Catalog No.:AB0109-100

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 300 µg

Description: Goat polyclonal antibody to TNF alpha. TNF-alpha is produced by a variety of immune cells including NK cells, B cells, T cells and macrophages. It is multifunctional proinflammatory cytokine that belongs to the tumour necrosis factor (TNF) superfamily. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is involved in the regulation of several processes including lipid metabolism cell differentiation and proliferation, coagulation and apoptosis. It has been implicated in a variety of diseases, including cancer and autoimmune diseases. 

Alternative names:
APC1, cachectin; tumor necrosis factor ligand superfamily member 2, macrophage-derived TNF, TNF-a, TNF monocyte-derived, tumor necrosis factor-alpha antibody.

Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant human TNF-alpha produced in E. coli.

Specificity: Detects a band of approximately 17 kDa using Raw264.7 cell line by Western blot.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                         1:500-1:2,000
Immunofluorescence                                  ND
Immunohistochemistry (paraffin)                ND
Immunohistochemistry (frozen)                  ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat +++ ND ND ND
mouse +++ ND ND ND
canine +++ ND ND ND
monkey +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.


For research use only, not for diagnostic use