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tdTomato Polyclonal Antibody

Catalog No.:AB8181-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength makes it ideal for live animal research.

Alternative names: Cherry fluorescent protein; DsRed, mCherry, red fluorescent protein, RFP antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Reactivity: Reacts against tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.

Usage: Western blot                              1:500-1:2,000
Immunofluorescence                                1:50-1:500
Immunohistochemistry (paraffin)              1:50-1:500
Immunohistochemistry (frozen)                1:50-1:500

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Coux RX, Teixeira FK, Lehmann R. Development. 2018 Apr. PMID:29511022
2. Weider M, Starost LJ, Groll K, et al. Nat Commun. 2018 Mar. PMID:29500351
3. Unnersjö-Jess D, Scott L, Sevilla SZ, et al. Kidney Int. 2017 Dec. PMID:29241621
4. Ishibashi F, Shimizu H, Nakata T, et al. Stem Cell Reports. 2017 Dec. PMID:29233556
5. Lacraz GPA, Junker JP, Gladka MM, et al. Circulation. 2017 Oct. PMID:28724751
6. Paolino A, Fenlon LR, Kozulin P, et al. J Neurosci Methods. 2017 Sep. PMID:28917658
7. Hurni N, Kolodziejczak M, Tomasello U, et al. Cereb Cortex. 2017 May. PMID:28334356
8. Beattie R, Postiglione MP, Burnett LE, et al. Neuron. 2017 May. PMID:28472654
9. Anzo M, Sekine S, Makihara S,  et al. Genes Dev. 2017 May. PMID:28637694




For research use only, not for diagnostic use