RFP Polyclonal Antibody

Catalog No.:AB1140-100

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 300 µg

Description: Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Alternative names:
Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant RFP peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Reactivity: Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato.

Usage: Western blot                             1:500-1:2,000
Immunofluorescence                               1:50-1:500
Immunohistochemistry (paraffin)             1:50-1:500
Immunohistochemistry (frozen)               1:50-1:500

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
Transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Tian DS, Peng J, Murugan M, et al. J Neurosci. 2017 Aug 16. PMID:28716963
2. Semerci F, Choi WT, Bajic A, et al. Elife. 2017 Jul 12. PMID:28699891



For research use only, not for diagnostic use