Product browse

News

New Distributor

Israel, Egypt, Turkey and other Middle East Countries BioTAG Ltd Read more

Distribution

Most of customers' orders are now dispatched directly from USA and GermanyRead more

RFP Polyclonal Antibody

Catalog No.:AB1140-100

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 300 µg

Description: Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Alternative names:
Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant RFP peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Reactivity: Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato.

Usage: Western blot                             1:500-1:2,000
Immunofluorescence                               1:50-1:500
Immunohistochemistry (paraffin)             1:50-1:500
Immunohistochemistry (frozen)               1:50-1:500

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
Transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. D Jiang, D Correa-Gallegos, S Christ, et al. Nat Cell Biol 2018 Apr. PMID:29593327
2. Tian DS, Peng J, Murugan M, et al. J Neurosci. 2017 Aug 16. PMID:28716963
2. Semerci F, Choi WT, Bajic A, et al. Elife. 2017 Jul 12. PMID:28699891



For research use only, not for diagnostic use