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Rab5a Polyclonal Antibody

Catalog No.:AB0009-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 800 µg

Description: Goat polyclonal antibody to mouse Rab5a. Rab5a belongs to the small GTPase superfamily, Rab family. Rab5a is a Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.

Alternative names: RAB5A, RAS-associated protein RAB5A, ras-related protein Rab-5A antibody.

Form: Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a produced in E. coli.

Specificity: Detects Rab5a protein by Western blot in cell lysates of transfected cells with GFP-Rab5a. This Ab is specific for Rab5a, it does not recognise Rab5b and Rab5c.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                           1:250-1:5,000
Immunofluorescence                             1:50-1:250
Immunohistochemistry (paraffin)                  ND
Immunohistochemistry (frozen)                    ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ ND ND
rat +++ +++ ND ND
mouse +++ +++ ND ND
canine +++ +++ ND ND
monkey +++ +++ ND ND

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References




For research use only, not for diagnostic use