Rab27a Polyclonal Antibody
Catalog No.:AB0045-200
Price / Unit: EUR 320.00
Source: Goat
Quantity/unit size: 400 µg
Description: Goat polyclonal antibody to mouse Rab27a. Rab27a belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.
Alternative names: GS2, GTP-binding protein Ram, rab-27, ras-related protein Rab-27A, RAB27, RAM.
Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli.
Specificity: Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody is specific for Rab27a. It does not recognise Rab27b protein.
Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.
Usage: Western blot 1:250-1:2,000
Immunofluorescence 1:50-1:200
Immunohistochemistry (paraffin) 1:50-1:400
Immunohistochemistry (frozen) 1:50-1:400
Reactivity chart
| Sample | Western Blot | Immuno-fluorescence | Immuno-Histochemistry (p) | Immuno-Histochemistry (f) | human | +++ | +++ | +++ | +++ | rat | +++ | +++ | +++ | +++ | mouse | +++ | +++ | +++ | +++ | canine | +++ | +++ | +++ | +++ | monkey | +++ | +++ | +++ | +++ |
|---|
Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.
References
1. Domingues L, Hurbain I, Gilles-Marsens F, et al. bioRxiv Jun 2019.
2. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018
For research use only, not for diagnostic use
