Rab27a Polyclonal Antibody

Catalog No.:AB0045-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal antibody to mouse Rab27a. Rab27a belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.

Alternative names: GS2, GTP-binding protein Ram, rab-27, ras-related protein Rab-27A, RAB27, RAM.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli.

Specificity: Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody is specific for Rab27a. It does not recognise Rab27b protein.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                            1:250-1:2,000
Immunofluorescence                              1:50-1:200
Immunohistochemistry (paraffin)            1:50-1:400
Immunohistochemistry (frozen)              1:50-1:400

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.


1. Domingues L, Hurbain I, Gilles-Marsens F, et al. bioRxiv Jun 2019.
2. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018

For research use only, not for diagnostic use