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Rab27a Polyclonal Antibody

Catalog No.:AB0023-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 500 µg

Description: Goat polyclonal antibody to mouse Rab27a. Rab27a belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.

Alternative names: GS2, GTP-binding protein Ram, rab-27, ras-related protein Rab-27A, RAB27, RAM.

Form: Polyclonal antibody supplied as a 500 µl (1 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a produced in E. coli.

Specificity: Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                             1:250-1:2,000
Immunofluorescence                               1:50-1:200
Immunohistochemistry (paraffin)             1:50-1:400
Immunohistochemistry (frozen)               1:50-1:400

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Pelkonen L, Reinisalo M, Morin-Picardat E et al. PLoS One. 2016 Aug 23, PMID: 27551967
2. Reichhart N, Markowski M, Ishiyama S et al. Cell Signal. 2015 Jul 31, PMID: 26235199



For research use only, not for diagnostic use