Rab27 Pan Polyclonal Antibody

Catalog No.:AB7223-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 1.5 mg

Description: Goat polyclonal antibody to mouse Rab27. Rab27 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.

Alternative names: C25KG, GS2, GTP-binding protein Ram, rab-27, Rab27a, Rab27b, ras-related protein Rab-27A, ras-related protein Rab-27B, RAB27, RAM antibody.

Form: Polyclonal antibody supplied as a 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptides derived from within residues 120 aa to the C-terminus of mouse Rab27a and Rab27b produced in E. coli.

Specificity: Detects total Rab27 levels by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a and GFP-Rab27b cds.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                        1:500-1:2,000
Immunofluorescence                          1:50-1:250
Immunohistochemistry (paraffin)        1:50-1:400
Immunohistochemistry (frozen)          1:50-1:400

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References




For research use only, not for diagnostic use