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P53 Polyclonal Antibody

Catalog No.:AB0154-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 800 µg

Description: Goat polyclonal antibody to P53. This protein reacts to diverse cellular stresses to regulate target genes that induce apoptosis, senescence, cell cycle arrest, DNA repair, or changes in metabolism. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is assumed to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumour suppressor.

Alternative names
: BCC7, LFS1 and TRP53 antibody.

Form: Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Recombinant peptide derived from within residues 280 aa to the C-terminus of human P53 produced in E. coli.

Specificity: Detects endogenous levels of P53 by Western blot and in transfected cells with GFP-P53.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                       1:500-1:5,000
Immunofluorescence                         1:25-1:250
Immunohistochemistry (paraffin)            ND
Immunohistochemistry (frozen)              ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ ND ND
rat +++ +++ ND ND
mouse +++ +++ ND ND
canine +++ +++ ND ND
monkey +++ +++ ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References




For research use only, not for diagnostic use