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Nucleolin Polyclonal Antibody

Catalog No.:AB0291-100

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 100 µg

Description: Goat polyclonal antibody to Nucleolin. NCL is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is found associated with pre-ribosomal particles and intranucleolar chromatin. May play a role in the process of transcriptional elongation and chromatin decondensation by binding to histone H1.

Alternative names: B530004O11Rik, C23, D0Nds28, D1Nds28, Nucl antibody.

Form: Polyclonal antibody supplied as a 100 µl (1 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli.

Specificity: Detects endogenous levels of NCL by Western blot.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                     1:1,000-1:5,000
Immunofluorescence                                ND
Immunohistochemistry (paraffin)              ND
Immunohistochemistry (frozen)                ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat +++ ND ND ND
mouse +++ ND ND ND
canine +++ ND ND ND
monkey +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References





For research use only, not for diagnostic use