Product browse

News

New Distributor

Israel, Egypt, Turkey and other Middle East Countries BioTAG Ltd Read more

Distribution

Most of customers' orders are now dispatched directly from USA and EuropeRead more

mCherry Polyclonal Antibody

Catalog No.:AB0081-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 1.5 mg

Description: Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Alternative names:
Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Form: Polyclonal antibody supplied as a 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody (AB0081) recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Reactivity: Red Fluorescent Protein (dsRed), tdTomato and mCherry.

Usage: Western blot                            1:500-1:5,000
Immunofluorescence                              1:50-1:500
Immunohistochemistry (paraffin)            1:50-1:500
Immunohistochemistry (frozen)              1:50-1:500

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
Transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Saito YC, Maejima T, Nishitani M, et al. J Neurosci. 2018 Jul. PMID:29915137
2. Raven AP, PhD Thesis, Edinburg University, United Kingdom 2018
3. Saito K, Nobuhisa I, Harada K et al. Exp Cell Res. 2018 Feb. PMID:29458175
4. Soya S, Takahashi TM, McHugh TJ, et al. Nat Commun. 2017 Nov. PMID:29151577
5. Fiuza M, Rostosky CM, Parkinson GT,et al. J Cell Biol. 2017 Aug. PMID:28855251
6. Hasegawa E, Maejima T, Yoshida T et al. Proc Natl Acad Sci U S A. 2017 Apr. PMID:28396432
7. Zhang-Hooks Y, Agarwal A, Mishina M, et al. Neuron. 2016 Jan. Supplemental Information, PMID:26774161
8. Stykel MG, MSc Thesis, University of Calgary 2016
9. Nakatsukasa K, Nishimura T, Byrne SD, et al. Mol Cell. 2015 Jul. Supplemental Information, PMID: 25982115
10. Julkowska MM, McLoughlin F, Galvan-Ampudia CS, et al. Plant Cell Environ. 2015 Mar, PMID: 25074439
11. Okorocha AE, PhD Thesis, University of Leicester, United Kingdom 2015
12. Déglon N, Merienne N, Genome editing for the treatment of huntington's disease, EP 2982758 A1 Patent, 2014
13. Benske A, MSc Thesis, The University of British Columbia, Canada 2014


For research use only, not for diagnostic use