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mCherry Polyclonal Antibody

Catalog No.:AB0081-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Alternative names:
Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody (AB0081) recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Reactivity: Red Fluorescent Protein (dsRed), tdTomato and mCherry.

Usage: Western blot                            1:500-1:5,000
Immunofluorescence                              1:50-1:500
Immunohistochemistry (paraffin)            1:50-1:500
Immunohistochemistry (frozen)              1:50-1:500

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Saito K, Nobuhisa I, Harada K et al. Exp Cell Res. 2018 Feb. PMID:29458175
2. Soya S, Takahashi TM, McHugh TJ, et al. Nat Commun. 2017 Nov. PMID:29151577
3. Fiuza M, Rostosky CM, Parkinson GT,et al. J Cell Biol. 2017 Aug. PMID:28855251
4. Hasegawa E, Maejima T, Yoshida T et al. Proc Natl Acad Sci U S A. 2017 Apr. PMID:28396432
5. Zhang-Hooks Y, Agarwal A, Mishina M,  et al. Neuron. 2016 Jan. Supplemental Information, PMID:26774161
6. Stykel MG, MSc Thesis, University of Calgary 2016
7. Nakatsukasa K, Nishimura T, Byrne SD, et al. Mol Cell. 2015 Jul. Supplemental Information, PMID: 25982115
8. Julkowska MM, McLoughlin F, Galvan-Ampudia CS, et al. Plant Cell Environ. 2015 Mar, PMID: 25074439
9. Déglon N, Merienne N, Genome editing for the treatment of huntington's disease, EP 2982758 A1 Patent, 2014



For research use only, not for diagnostic use