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Human IgG Polyclonal Antibody

Catalog No.:AB0147-200

Price / Unit: EUR 300.00

Source: Goat

Quantity/unit size: 800 µg

Description: Goat polyclonal antibody to human IgG. IgG is a monomeric immunoglobulin, with two heavy chains and two light chains. IgG can be found in blood and is the most abundant immunoglobulin, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses (IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%)) that can bind to many types of pathogens, protecting the body against them by complement activation, opsonization for phagocytosis and neutralisation of their toxins.

Alternative names
: Immunoglobulin G antibody.

Form: Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Full IgG affinity purified from serum of four human donors tested and found negative for Hepatitis B, Hepatitis C, HIV I and II antibodies using protein G. Purity >95% by SDS-PAGE.

Specificity: Using serum samples detects the light and heavy chains by Western blot.

Reactivity: Reacts against human.

Usage: Western blot                          1:500-1:2,000
Immunofluorescence                                  ND
Immunohistochemistry (paraffin)                ND
Immunohistochemistry (frozen)                  ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat ND ND ND ND
mouse ND ND ND ND
canine ND ND ND ND
monkey ND ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References





For research use only, not for diagnostic use