HIF1 alpha Polyclonal Antibody

Catalog No.:AB0070-100

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 300 µg

Description: Goat polyclonal to alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in apoptosis, angiogenesis, energy metabolism, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in tumour angiogenesis, embryonic vascularization, and pathophysiology of ischemic disease.

Alternative names:
alpha subunit (basic helix-loop-helix transcription factor), ARNT interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF-1alpha, HIF1, HIF1-ALPHA, HIF1a, hypoxia inducible factor 1, hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, hypoxia-inducible factor 1-alpha, member of PAS protein 1, member of PAS superfamily 1, MOP1, PASD8, PAS domain-containing protein 8 antibody.

Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Recombinant peptide derived from within residues 470 to 590 aa of human HIF1a produced in E. coli.

Specificity: Detects HIF1a in transfected cells and a band of approximately 120 kDa by Western blot in cell lysates.

Reactivity: Reacts against human, canine and mouse proteins.

Usage: Western blot                        1:500-1:2,000
Immunofluorescence                                  ND
Immunohistochemistry (paraffin)                ND
Immunohistochemistry (frozen)                  ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat +++ ND ND ND
mouse +++ ND ND ND
canine +++ ND ND ND
monkey +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Ramalho AR, Toscano A, Pereira P. et al. Rev Port Cardiol. 2017 May 4. PMID:28479269



For research use only, not for diagnostic use