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GST Polyclonal Antibody

Catalog No.:AB9929-500

Price / Unit: EUR 420.00

Source: Goat

Quantity/unit size: 1.5 mg

Description: Goat polyclonal antibody to GST (Glutathione-S-Transferase). GST is a 26 kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in GST plasmid expression vectors as a fusion protein with foreign protein.

Form: Polyclonal antibody supplied as a 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant protein produced in E. coli.

Specificity: In lysates of E. coli cells transformed with pGEX plasmid detects a band of 26 kDa by Western blot.

Reactivity: Reacts with Schistosoma japonicum. Not yet tested on other species.

Usage: Western blot                         1:500-1:2,000
Immunofluorescence                                  ND
Immunohistochemistry (paraffin)                ND
Immunohistochemistry (frozen)                  ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
Transformed cells +++ ND ND ND

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Domingues L, Ismail A, Charro N,  et al. Cell Microbiol. 2016 Jul;18(7):949-69. PMID: 26676327.



For research use only, not for diagnostic use