GFP Polyclonal Antibody

Catalog No.:AB0066-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.

Alternative names:
Green fluorescent protein antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognise mCherry fluorescent protein.


Usage: Western blot                              1:500-1:5,000
Immunofluorescence                               1:50-1:1,000
Immunohistochemistry (paraffin)             1:50-1:1,000
Immunohistochemistry (frozen)               1:50-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.


1. Schlöffel, MA, PhD Thesis, University of Tübingen, Germany 2020
2. Zhang Y, Tang N, Luo J, et al. J Virol 2019 Jun. PMID:31189706
3. Meyers A, Furtmann C, Gesing K, et al. Microb Biotechnol 2019 Jun.PMID:31237428
4. Anjo SI, Melo MN, Loureiro LR, et al. Redox Biol 2019 Apr. PMID:30737169
5. Vicente MM, Mendes A, Cruz M, et al. bioRxiv 555144; Feb 2019
6. Gassama A, PhD Thesis, Zurich University, Swizterland 2018
7. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018
8. Almeida F, Luís MP, Pereira IS, et al. Front Cell Infect Microbiol 2018 Jul. PMID30094225
9. Radtke C and Tews BA. J Gen Virol 2017 Oct. PMID:28874234
10. Wei-Lin Wan, PhD Thesis, University of Tubingen, Germany 2017
11. Tiede C, Bedford R, Heseltine SJ, et al. Elife. 2017 Jun. PMID:28654419
12. Gabriel SS, Belge H, Gassama A, et al. Kidney Int 2017 Apr. PMID:28143656
13. Krishnamurthy VV, Khamo JS, Mei W, et al. Development 2016 Nov. PMID: 27697903
14. Zhang-Hooks Y, Agarwal A, Mishina M, et al. Neuron 2016 Jan (Supplemental Information). PMID:26774161
15. Carneiro L, Geller S, Fioramonti X, et al. Am J Physiol Endocrinol Metab 2016 Jan. PMID:26530151
16. Roux I, Wu JS, McIntosh JM, et al. J Neurophysiol 2016 Apr. PMID: 27098031
17. Bugalhão JN, Mota LJ, Franco IS. Microbiologyopen 2015 Dec. PMID:26626407
18. Sargiannidou I, Kim GH, Kyriakoudi S, et al. Neurogenetics 2015 Mar. PMID: 25771809
19. Issa JB, Haeffele BD, Agarwal A, et al. Neuron 2014 Aug (Supplemental Information). PMID:25088366
20. Moreiras HAF, MSc Thesis, University of Lisbon, Portugal 2014

For research use only, not for diagnostic use