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GFP Polyclonal Antibody

Catalog No.:AB0066-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to GFP (green fluorescent protein).

Alternative names:
Green fluorescent protein antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognise mCherry fluorescent protein.

Reactivity:

Usage: Western blot                              1:500-1:5,000
Immunofluorescence                               1:50-1:1,000
Immunohistochemistry (paraffin)             1:50-1:1,000
Immunohistochemistry (frozen)               1:50-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Tiede C, Bedford R, Heseltine SJ, et al. Elife. 2017 Jun. PMID:28654419
2. Gabriel SS, Belge H, Gassama A, et al. Kidney Int. 2017 Apr. PMID:28143656
3. Krishnamurthy VV, Khamo JS, Mei W, et al. Development. 2016 Nov. PMID: 27697903
4. Zhang-Hooks Y, Agarwal A, Mishina M,  et al. Neuron. 2016 Jan (Supplemental Information). PMID:26774161
5. Carneiro L, Geller S, Fioramonti X, et al. Am J Physiol Endocrinol Metab. 2016 Jan. PMID:26530151
6. Roux I, Wu JS, McIntosh JM, et al. J Neurophysiol. 2016 Apr. PMID: 27098031
7. Bugalhão JN, Mota LJ, Franco IS. Microbiologyopen. 2015 Dec. PMID:26626407
8. Sargiannidou I, Kim GH, Kyriakoudi S, et al. Neurogenetics. 2015 Mar. PMID: 25771809
9. Issa JB, Haeffele BD, Agarwal A, et al. Neuron. 2014 Aug (Supplemental Information). PMID:25088366




For research use only, not for diagnostic use