GFP Polyclonal Antibody

Catalog No.:AB0020-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 1 mg

Description: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.


Usage: Western blot                            1:500-1:5,000
Immunofluorescence                              1:50-1:1,000
Immunohistochemistry (paraffin)            1:50-1:1,000
Immunohistochemistry (frozen)              1:50-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.


1. Fukuda S, Kaneshige A, Kaji T, et al. Elife. 2019 Sep. PMID:31545169
2. Matsuda T, Oinuma I. Sci Rep. 2019 Aug. PMID:31383899
3. Geller S, Arribat Y, Netzahualcoyotzi C, et al. Cell Metab. 2019 Aug. PMID:31474567
4. Zhang L, Uezumi A, Kaji T, et al. Int J Mol Sci. 2019 Jul. PMID:31277245
5. Alenquer M, Vale-Costa S, Etibor TA, et al. Nat Commun. 2019 Apr. PMID:30967547
6. Koike M, Yutoku Y, Koike A. FEBS Open Bio. 2019 Jan. PMID:31115163
5. Vyas P, Wu JS, Jimenez A, et al. Sci Rep. 2019 Apr. PMID:30944354
8. Alenquer M, Vale-Costa S, Sousa AL, et al. bioRxiv 410373; Sept 2018
9. Larson VA, Mironova Y, Vanderpool KG, et al. Elife. 2018 Mar. PMID:29596047
10. Festa BP, Chen Z, Berquez M, et al. Nat Commun. 2018 Jan. PMID:29323117
11. Ramos-Nascimento A, Kellen B, Ferreira F, et al. J Cell Sci. 2017 Dec. PMID:29061883
12. Wu JS, Vyas P, Glowatzki E, et al. J Comp Neurol. 2017 Oct. PMID:29055051
13. Agarwal A, Wu PH, Hughes EG et al. Neuron. 2017. PMID:28132831
14. Mimura Y, Takagi M, Clever M et al. J Cell Sci. 2016 Nov 15. PMID:27802161
15.Vyas P, Wu JS, Zimmerman A et al. J Assoc Res Otolaryngol. 2016 Sep. PMID:27696081
16.Vale-Costa S, Alenquer M, Sousa AL et al. J Cell Sci. 2016 Mar. PMID:26940915
17. Martins-Marques T, Anjo SI, Pereira P et al. Mol Cell Proteomics. 2015 Aug. PMID:26316108 Supplemental Information
18. Sargiannidou I, Kim GH, Kyriakoudi S et al. Neurogenetics. 2015 Jul. PMID: 25771809
19. Kourti M, Ikonomou G, Giakoumakis NN et al. Cell Signal. 2015 Mar. PMID: 25744540
20. Monteiro NCO, MSc Thesis, University of Coimbra, Portugal 2015
21. Ferreira RRS, MSc Thesis, University of Coimbra, Portugal 2015
22. Paukert M, Agarwal A, Cha J et al. Neuron. 2014 Jun. PMID: 24945771 Supplemental Information
23. Moreiras HAF, MSc Thesis, University of Lisbon, Portugal 2014
24. Pais SV, Milho C, Almeida F et al. PLoS One. 2013. PMID: 23431368

For research use only, not for diagnostic use