GFP Polyclonal Antibody

Catalog No.:AB0066-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 1.5 mg

Description: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.

Alternative names: Green fluorescent protein antibody.

Form: Polyclonal antibody supplied as a 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.

Reactivity:

Usage: Western blot                         1:500-1:5,000
Immunofluorescence                           1:50-1:1,000
Immunohistochemistry (paraffin)         1:50-1:1,000
Immunohistochemistry (frozen)           1:50-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

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6. Wei-Lin Wan, PhD Thesis, University of Tubingen, Germany 2017
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16. Moreiras HAF, MSc Thesis, University of Lisbon, Portugal 2014



For research use only, not for diagnostic use