GFP Polyclonal Antibody

Catalog No.:AB0020-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.

Reactivity:

Usage: Western blot                             1:500-1:5,000
Immunofluorescence                              1:50-1:1,000
Immunohistochemistry (paraffin)            1:50-1:1,000
Immunohistochemistry (frozen)              1:50-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

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9.Vyas P, Wu JS, Zimmerman A et al. J Assoc Res Otolaryngol. 2016 Sep. PMID:27696081
10.Vale-Costa S, Alenquer M, Sousa AL et al. J Cell Sci. 2016 Mar. PMID:26940915
11. Martins-Marques T, Anjo SI, Pereira P et al. Mol Cell Proteomics. 2015 Aug. PMID:26316108 Supplemental Information
12. Sargiannidou I, Kim GH, Kyriakoudi S et al. Neurogenetics. 2015 Jul. PMID: 25771809
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14. Monteiro NCO, MSc Thesis, University of Coimbra, Portugal 2015
15. Ferreira RRS, MSc Thesis, University of Coimbra, Portugal 2015
16. Paukert M, Agarwal A, Cha J et al. Neuron. 2014 Jun. PMID: 24945771 Supplemental Information
17. Moreiras HAF, MSc Thesis, University of Lisbon, Portugal 2014
18. Pais SV, Milho C, Almeida F et al. PLoS One. 2013. PMID: 23431368



For research use only, not for diagnostic use