GFP Polyclonal Antibody

Catalog No.:AB0020-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal antibody to GFP (green fluorescent protein).

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.

Reactivity:

Usage: Western blot                             1:500-1:5,000
Immunofluorescence                              1:50-1:1,000
Immunohistochemistry (paraffin)            1:50-1:1,000
Immunohistochemistry (frozen)              1:50-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Festa BP, Chen Z, Berquez M, et al. Nat Commun. 2018 Jan. PMID:29323117
2. Ramos-Nascimento A, Kellen B, Ferreira F, et al. J Cell Sci. 2017 Dec. PMID:29061883
3. Wu JS, Vyas P, Glowatzki E, et al. J Comp Neurol. 2017 Oct. PMID:29055051
4. Agarwal A, Wu PH, Hughes EG et al. Neuron. 2017. PMID:28132831
5. Mimura Y, Takagi M, Clever M et al. J Cell Sci. 2016 Nov 15. PMID:27802161
6.Vyas P, Wu JS, Zimmerman A et al. J Assoc Res Otolaryngol. 2016 Sep. PMID:27696081
7.Vale-Costa S, Alenquer M, Sousa AL et al. J Cell Sci. 2016 Mar. PMID:26940915
8. Martins-Marques T, Anjo SI, Pereira P et al. Mol Cell Proteomics. 2015 Aug. PMID:26316108 Supplemental Information
9. Sargiannidou I, Kim GH, Kyriakoudi S et al. Neurogenetics. 2015 Jul. PMID: 25771809
10. Kourti M, Ikonomou G, Giakoumakis NN et al. Cell Signal. 2015 Mar. PMID: 25744540
11. Paukert M, Agarwal A, Cha J et al. Neuron. 2014 Jun. PMID: 24945771 Supplemental Information
12. Pais SV, Milho C, Almeida F et al. PLoS One. 2013. PMID: 23431368



For research use only, not for diagnostic use