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GAPDH Polyclonal Antibody

Catalog No.:AB0067-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Alternative names: glyceraldehyde 3-phosphate dehydrogenase,  glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Specificity: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                                  1:500-1:2,000
Immunofluorescence                                    1:50-1:250
Immunohistochemistry (paraffin)                1:200-1:1,000
Immunohistochemistry (frozen)                  1:200-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Sacchi V, Wang BJ, Kubli D, et al. J Am Heart Assoc. 2017 Oct (Supplemental Information). PMID:29018025
2. Khalafalla FG, Kayani W, Kassab A, et al. J Physiol. 2017 Oct. PMID:28980705
3. Firouzi F, MSc Thesis, San Diego State University 2017
4. Gonçalves A, Lin CM, Muthusamy A, et al. Invest Ophthalmol Vis Sci. 2016 May. PMID: 27163772
5. Hyenne V, Apaydin A, Rodriguez D, et al. J Cell Biol. 2015 Oct. PMID: 26459596
6. Helsby MA, Leader PM, Fenn JR. BMC Cell Biol. 2014 Feb. PMID: 24528853
7. Grilo MLJ, MSc Thesis, University of Coimbra 2014
8. Matos CTSM, PhD Thesis, NOVA University of Lisbon 2013



For research use only, not for diagnostic use