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GAPDH Polyclonal Antibody

Catalog No.:AB0049-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 1 mg

Description: Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Alternative names: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Specificity: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates

Reactivity: Reacts against human, rat, mouse, zebrafish, canine and monkey proteins.

Usage: Western blot                            1:500-1:5,000
Immunofluorescence                              1:50-1:250
Immunohistochemistry (paraffin)          1:200-1:1,000
Immunohistochemistry (frozen)            1:200-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
zebrafish +++ ND ND ND
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Ribeiro ST, Tesio M, Ribot JC, et al. Leukemia. 2017 Jul. PMID:27899804
2. Santarino IB, Viegas MS, Domingues NS, et al. Sci Rep. 2017 Jul. PMID:28724916
3. Ribeiro STF, PhD Thesis, University of Lisbon, Portugal 2017 4. Robinson CL, Evans RD, Briggs DA, et al. J Cell Sci. 2017 May. PMID:28490438
5. Ramalho AR, Toscano A, Pereira P. et al. Rev Port Cardiol. 2017 May. PMID:28479269
6. Vale-Costa S, Alenquer M, Sousa AL, et al. J Cell Sci. 2016 Mar. PMID:26940915
7. Encarnação M, Espada L, Escrevente C, et al. J Cell Biol. 2016 Jun. PMID: 27325790
8. Ferreira JV, Soares AR, Ramalho JS, et al. Scientific Reports 5, May 2015. PMID:25958982
9. Ferreira RRS, MSc Thesis, University of Coimbra, Portugal 2015
10.Paiva RA, MSc Thesis, University of Coimbra, Portugal 2015 11. Casalou C, Seixas C, Portelinha A, et al. J Cell Sci. 2014 Jun. PMID:24777479
12. Ribeiro-Rodrigues TM, Catarino S, Marques C, et al. FASEB J. 2014 Nov. PMID:25070368
13. Moreiras HAF, MSc Thesis, University of Lisbon, Portugal 2014


For research use only, not for diagnostic use