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Firefly Luciferase Polyclonal Antibody

Catalog No.:AB0131-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 1.5 mg

Description: Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Alternative names: Luc and Luciferin 4-monooxygenase antibody.

Form: Polyclonal antibody supplied as a 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot.

Reactivity: Firefly (Photinus pyralis)

Usage: Western blot                          1:250-1:2,000
Immunofluorescence                                  ND
Immunohistochemistry (paraffin)                ND
Immunohistochemistry (frozen)                  ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
transfected cells +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References



For research use only, not for diagnostic use