EEA1 Polyclonal Antibody
Catalog No.:AB0005-200
Price / Unit: EUR 320.00
Source: Goat
Quantity/unit size: 600 µg
Description: Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
Alternative names: Early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2 antibody.
Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Immunogen: Purified recombinant peptide within residues 1,230 aa to the C-terminus of human EEA1 produced in E. coli.
Specificity: Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.
Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.
Usage: Western blot 1:250-1:1,000
Immunofluorescence 1:50-1:250
Immunohistochemistry (paraffin) ND
Immunohistochemistry (frozen) ND
Reactivity chart
| Sample | Western Blot | Immuno-fluorescence | Immuno-Histochemistry (p) | Immuno-Histochemistry (f) | human | +++ | +++ | ND | ND | rat | +++ | +++ | ND | ND | mouse | +++ | +++ | ND | ND | canine | +++ | +++ | ND | ND | monkey | +++ | +++ | ND | ND |
|---|
Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.
References
1. Beck MCG, PhD Thesis, University of Ulm, Germany 2018
For research use only, not for diagnostic use
