EEA1 Polyclonal Antibody

Catalog No.:AB0005-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.

Alternative names: Early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2 antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide within residues 1,230 aa to the C-terminus of human EEA1 produced in E. coli.

Specificity: Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                            1:250-1:1,000
Immunofluorescence                              1:50-1:250
Immunohistochemistry (paraffin)                  ND
Immunohistochemistry (frozen)                    ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ ND ND
rat +++ +++ ND ND
mouse +++ +++ ND ND
canine +++ +++ ND ND
monkey +++ +++ ND ND

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References



For research use only, not for diagnostic use