Clathrin HC Polyclonal Antibody

Catalog No.:AB0059-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal to Clathrin Heavy Chain. Clathrin is formed by three clathrin heavy chains and three light chains. This protein is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in endocytosis of a variety of macromolecules and in the intracellular trafficking of receptors.

Alternative names: clathrin, clathrin heavy chain 1, clathrin heavy polypeptide (Hc), clathrin heavy polypeptide-like 2, CLTC, CHC17, CLH-17, CLTCL2, Hc, heavy chain (Hc) antibody.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within residues 50 aa to N-terminus of human CLTC produced in E. coli.

Specificity: Detects endogenous levels of total CLTC by Western blot in the whole cell lysates (MDCK, At-T20, COS-7).

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                            1:250-1:1,000
Immunofluorescence                              1:25-1:250
Immunohistochemistry (paraffin)                 ND
Immunohistochemistry (frozen)                   ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ ND ND
rat +++ +++ ND ND
mouse +++ +++ ND ND
canine +++ +++ ND ND
monkey +++ +++ ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

Caì Y, Postnikova EN, Bernbaum JG, et al.  J Virol. 2015 Jan 1;89(1):844-56.



For research use only, not for diagnostic use