CD4 Polyclonal Antibody

Catalog No.:AB0091-200

Price / Unit: EUR 300.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to CD4. CD4 is a member of the immunoglobulin superfamily, a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and a receptor for the human immunodeficiency virus. This protein is expressed not only on the surface of T helper cells and regulatory T cells but also on monocytes, macrophages and dendritic cells. It functions to initiate or amplify the early phase of T-cell activation and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Alternative names: CD4mut, CD4 antigen (p55), CD4 Receptor , T-cell surface antigen T4/Leu-3 and T-cell surface glycoprotein CD4 antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within 50 to 235 aa, corresponding to the external domain of human CD4 produced in E. coli.

Specificity: Using spleen lysate detects a 52 kDa band by Western blot.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                            1:500-1:2,000
Immunofluorescence                                   ND
Immunohistochemistry (paraffin)                 ND
Immunohistochemistry (frozen)                   ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat +++ ND ND ND
mouse +++ ND ND ND
canine +++ ND ND ND
monkey +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Helsby MA, Leader PM, Fenn JR, et al. BMC Cell Biol. 2014 Feb. PMID:24528853



For research use only, not for diagnostic use