CD4 Polyclonal Antibody

Catalog No.:AB0091-200

Price / Unit: EUR 300.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to CD4. CD4 is a member of the immunoglobulin superfamily, a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and a receptor for the human immunodeficiency virus. This protein is expressed not only on the surface of T helper cells and regulatory T cells but also on monocytes, macrophages and dendritic cells. It functions to initiate or amplify the early phase of T-cell activation and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Alternative names: CD4mut, CD4 antigen (p55), CD4 Receptor , T-cell surface antigen T4/Leu-3 and T-cell surface glycoprotein CD4 antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide derived from within 50 to 235 aa, corresponding to the external domain of human CD4 produced in E. coli.

Specificity: Using spleen lysate detects a 52 kDa band by Western blot.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                            1:500-1:2,000
Immunofluorescence                                   ND
Immunohistochemistry (paraffin)                 ND
Immunohistochemistry (frozen)                   ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ ND ND ND
rat +++ ND ND ND
mouse +++ ND ND ND
canine +++ ND ND ND
monkey +++ ND ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Helsby MA, Leader PM, Fenn JR, Gulsen T, Bryant C, Doughton G, Sharpe B, Whitley P, Caunt CJ, James K, Pope AD, Kelly DH, Chalmers AD. BMC Cell Biol. 2014 Feb 14;15:6. PMID:24528853



For research use only, not for diagnostic use