Calnexin Polyclonal Antibody

Catalog No.:AB0037-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker. CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Alternative names: Calnexin, CALX, CNX, FLJ26570,  histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Specificity: Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118,  rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Reactivity: Reacts against human, rat, mouse, monkey and canine proteins.

Usage: Western blot                              1:500-1:5,000
Immunofluorescence                               1:50-1:500
Immunohistochemistry (paraffin)           1:200-1:1,000
Immunohistochemistry (frozen)             1:200-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Neves C, Rodrigues T, Sereno J, et al. Oxid Med Cell Longev. 2019 Jun. PMID:31341532
2. Silva MM, Gomes-Alves P, Rosa S, et al. J Biotechnol. 2018 Aug. PMID:30165116
3. Ribeiro M, Castelhano J, Petrella LI, et al. J Magn Reson Imaging. 2018 Jan. PMID:29377412
4. Ribeiro STF, PhD Thesis University of Lisbon, Portugal 2017
5. Rodrigues T, Matafome P, Sereno J, et al. Sci Rep. 2017 May 10;7:1698. PMID:28490763
6. Cabral AMD, MSc Thesis, University of Lisbon 2017
7. Thieleke-Matos C, Lopes da Silva M, Cabrita-Santos L et al. Cellular Microbiology 2016;18:437-50. PMID: 26399761
8. Sivadasan R, PhD Thesis, Wurzburg University 2016
9. Neves CAF, MSc Thesis University of Aveiro, Portugal 2015




For research use only, not for diagnostic use