Calnexin Polyclonal Antibody

Catalog No.:AB0041-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 400 µg

Description: Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker. CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Alternative names: Calnexin, CALX, CNX, FLJ26570,  histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.

Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Specificity: Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118,  rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Reactivity: Reacts against human, rat, mouse, monkey and canine proteins.

Usage: Western blot                              1:500-1:5,000
Immunofluorescence                               1:50-1:500
Immunohistochemistry (paraffin)           1:200-1:1,000
Immunohistochemistry (frozen)             1:200-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
rat +++ +++ +++ +++
mouse +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long term storage and 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Pelkonen L, Sato K, Reinisalo M, et al. Mol Pharm. 2017 Mar 6. PMID:28112518
2. Martins-Marques T, Pinho MJ, Zuzarte M. et al. J Extracell Vesicles. 2016 Sep 29;5:32538. PMID:27702427
3. Ferreira RRS, MSc Thesis, Coimbra University 2015
4. Paiva RA, MSc Thesis, Coimbra University 2015
5. Samtleben S, PhD Thesis, Universität Würzburg 2014
6. Ribot JC, Ribeiro ST, Correia DV, Sousa AE, Silva-Santos B. J Immunol. 2014 Mar, PMID: 24489097
7. Marques, CIR, MSc Thesis, Coimbra University 2013


For research use only, not for diagnostic use

*This antibody is identical to AB4100.