Calnexin Polyclonal Antibody

Catalog No.:AB0037-500

Price / Unit: EUR 450.00

Source: Goat

Quantity/unit size: 1 mg

Description: Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker. CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Alternative names: Calnexin, CALX, CNX, FLJ26570,  histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.

Form: Polyclonal antibody supplied as a 500 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Specificity: Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                              1:500-1:5,000
Immunofluorescence                               1:50-1:500
Immunohistochemistry (paraffin)           1:200-1:1,000
Immunohistochemistry (frozen)             1:200-1:1,000

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ +++ +++
mouse +++ +++ +++ +++
rat +++ +++ +++ +++
canine +++ +++ +++ +++
monkey +++ +++ +++ +++

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Monteiro-Alfredo T, Matafome P, Iacia BP, et al. Oxid Med Cell Longev 2020 Mar. PMID: 32256951
2. Fricke S, Metzdorf K, Ohm M, et al. Cell Rep. 2019 Oct. PMID:31618635
3. Neves C, Rodrigues T, Sereno J, et al. Oxid Med Cell Longev 2019 Jun. PMID:31341532
4. Fonseca LMO, MSc Thesis, University of Coimbra, Portugal 2018
5. Silva MM, Gomes-Alves P, Rosa S, et al. J Biotechnol. 2018 Aug. PMID:30165116
6. Rodrigues TDA, PhD Thesis, University of Coimbra, Portugal 2018
7. Ribeiro M, Castelhano J, Petrella LI, et al. J Magn Reson Imaging 2018 Jan. PMID:29377412
8. Awadh A, PhD Thesis, University of Alberta, Canada 2018
9. Ribeiro STF, PhD Thesis University of Lisbon, Portugal 2017
10. Rodrigues T, Matafome P, Sereno J, et al. Sci Rep. 2017 May. PMID:28490763
11. Cabral AMD, MSc Thesis, University of Lisbon 2017
12. Thieleke-Matos C, Lopes da Silva M, Cabrita-Santos L et al. Cellular Microbiology 2016. PMID: 26399761
13. Sivadasan R, PhD Thesis, Wurzburg University 2016
14. Neves CAF, MSc Thesis University of Aveiro, Portugal 2015




For research use only, not for diagnostic use