Product browse

News

New Distributor

Israel, Egypt, Turkey and other Middle East Countries BioTAG Ltd Read more

Distribution

Most of customers' orders are now dispatched directly from USA and EuropeRead more

beta-Actin Polyclonal Antibody

Catalog No.:AB0145-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Actin proteins are highly conserved and ubiquitously expressed in all eukaryotic cells. These proteins are the major components of the cytoskeleton and involved in various types of cell motility.

Alternative names:
ACTB, beta-actin, BRWS1, PS1TP5BP1 antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Recombinant peptide derived from within residues 100 aa to the N-terminus of human beta-Actin produced in E. coli.

Specificity: Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Reactivity: Reacts against human, rat, mouse, monkey and canine proteins.

Usage: Western blot                      1:250-1:2,000
Immunofluorescence                          1-50-1:250
Immunohistochemistry (paraffin)               ND
Immunohistochemistry (frozen)                 ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ ND ND
rat +++ +++ ND ND
mouse +++ +++ ND ND
canine +++ +++ ND ND
monkey +++ +++ ND ND

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References



For research use only, not for diagnostic use