ATG12 Polyclonal Antibody

Catalog No.:AB0083-200

Price / Unit: EUR 320.00

Source: Goat

Quantity/unit size: 600 µg

Description: Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

Alternative names: APG12, Apg12 (autophagy, yeast) homolog, APG12L, FBR93, HAPG12,  autophagy protein 12, autophagy-related protein 12 antibody.

Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Immunogen: Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.

Specificity: Detects GFP-ATG12 in transfected cells by Western blot.

Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.

Usage: Western blot                           1:250-1:2,000
Immunofluorescence                             1:50-1:250
Immunohistochemistry (paraffin)                ND
Immunohistochemistry (frozen)                  ND

Reactivity chart

Sample Western Blot Immuno-fluorescence Immuno-Histochemistry (p) Immuno-Histochemistry (f)
human +++ +++ ND ND
rat +++ +++ ND ND
mouse +++ +++ ND ND
canine +++ +++ ND ND
monkey +++ +++ ND ND

Storage: Store at -20 C for long term storage. Store at 2-8 C for up to one month.

Special instructions: Avoid freeze/thaw cycles.

References

1. Kharaziha P, Panaretakis T. Methods Enzymol. 2017;587:247-255. PMID:28253959



For research use only, not for diagnostic use