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Package 5 - protein provided by customer
Description
  • Immunization using 1 goat
  • Pre-immune serum (5-10 ml)
  • 5 immunizations (1, 3, 5, 7, 9th week)
  • 4 serum bleeding (5, 7, 9, 12th week)
  • 500-600 ml antiserum per animal (or 500-1000 ml more *)
  • ELISA test (until titer reaches 20,000)
  • Optional Western blotting test available
  • Serum separation
  • 12 weeks protocol period
No of Animals 1
Protein/peptide Required
  • Customer provide 1-2 mg protein (purity : 90-95%)**
  • Antigen information (protein name, GenBank Acc n., MW, toxicity , buffer, concentration etc.
Pre-immune serum Yes
Conjugation included Yes
ELISA testing ELISA test (until titer reaches >20,000)
Western Blot Optional
Purification Optional
  • Protein G purification
  • Antigen-affinity purification
Antibody to Customer 500-600 ml antiserum per animal*
Notes *If the customer decides not to continue the project, extra 500-1000 ml of serum will be
obtained by terminal bleed; this option might not be available
** If affinity purification is performed, at least an additional of 1-2 mg of protein will required