Site-directed mutagenesis

SICGEN  can perform site-specific mutation in any double-stranded plasmid. Different types of mutations (point mutations, switch amino acids, deletions or insertions of single or multiple amino acids) are available.

The service includes:

- Design and synthesis of oligonucleotides
- DNA mutagenesis (multiple mutations within 24-nucleotide proximity in one reaction)
- Sequencing of the target DNA in one direction
- 5-10 µg of sequencing verified plasmid containing the gene of interest and/or a bacteria-stub and the final sequencing data will delivered

Customer provides:

- Template DNA plasmid
- Information about the template/vector
- Mutation information to be made

Cat. No Service Details Unit Price Order
CS0150 Site-directed mutagenesis (1-2 reactions/construct) 300.00 orders@sicgen.pt
CS0151 Site-directed mutagenesis (1-2 reactions/construct) 400.00 orders@sicgen.pt
CS0152 Site-directed mutagenesis (3-5 reactions/construct) 250.00 orders@sicgen.pt
CS0153 Site-directed mutagenesis (3-5 reactions/construct) 350.00 orders@sicgen.pt
CS0154 Site-directed mutagenesis (≥5 reactions/construct) 200.00 orders@sicgen.pt
CS0155 Site-directed mutagenesis (≥5 reactions/construct) 300.00 orders@sicgen.pt