Product browse

News

New Distributor

Israel, Egypt, Turkey and other Middle East Countries BioTAG Ltd Read more

Distribution

Most of customers' orders are now dispatched directly from USA and EuropeRead more

cDNA/DNA PCR cloning

SICGEN performs PCR and RT-PCR cloning of known genes from DNA/RNA source. PCR product will be cloned into a basic cloning vector or other vector provided or authorised by the customer. The resulting cloned products are then confirmed by direct sequencing.

The service includes:

- Design and synthesis of oligonucleotides (if applicable)
- DNA amplification with high proof reading polymerase
- Sequencing of the target DNA in one direction
- 5-10 µg of sequencing verified plasmid containing the gene of interest and the final sequencing data will delivered

Customer provides:

- Information about the template and cloning vector
- Template DNA
- Specific cloning vector

Cat. No Service Details Unit Price Order
CS0120 DNA Cloning (< 500 bp) 200.00 orders@sicgen.pt
CS0121 DNA Cloning (< 500 bp) 300.00 orders@sicgen.pt
CS0122 DNA Cloning (< 500 bp) 150.00 orders@sicgen.pt
CS0123 DNA Cloning (< 500 bp) 250.00 orders@sicgen.pt
CS0124 DNA Cloning (501 bp -1.5 kb) 300.00 orders@sicgen.pt
CS0125 DNA Cloning (501 bp -1.5 kb) 0.00 orders@sicgen.pt
CS0126 DNA Cloning (501 bp -1.5 kb) 250.00 orders@sicgen.pt
CS0127 DNA Cloning (501 bp -1.5 kb) 0.00 orders@sicgen.pt
CS0128 DNA Cloning (≥1.5 kb) 0.00 orders@sicgen.pt
CS0129 DNA Cloning (≥1.5 kb) 0.00 orders@sicgen.pt